Department of Molecular Probes and Prodrugs

dr Jacek Ł. Kolanowski

Dr. Jacek Ł. Kolanowski

Head of Department

jacek.kolanowski@ibch.poznan.pl
ext. 1165

Researchers:

Dr. Dorota Jakubczyk
adiunkt

Dr. Michał Jakubczyk
adiunkt

Dr. Dorota Kwiatek
adiunkt

Dr. Magdalena Derbis
asystent

Research Projects

Research area

The main aim is to develop low-molecular weight chemical tools to image, study and control biological systems through mapping and subsequently modifying interactions between biochemical targets in live cells (figure). In particular:

  • Fluorescent and bioluminescent molecular probes for sensing pairs of analytes to associate biochemical changes inside cells with the development of disease (e.g. lung and prostate cancer).
  • Non-genetic methods for in situ tagging of endogenous proteins to visualise endogenous proteins and study their interactions in live cells.
  • Multiplexing assays (based on small molecules) for high-throughput screening to improve the identification of most promising small molecule drug candidates and deciphering their mechanism of action (also within the ERIC EU-Openscreen consortium and POL-Openscreen initiative)
  • molecular tools for super-resolution new generation fluorescent nanoscopy (resolution approx.. 1 nm) in live biological models
  • development of molecular tools and assays to study mechanisms of infection and searching for therapy against COVID-19

These tasks encompass, among others:

  • Organic synthesis of probes (low molecular weight fluorophores and bioluminophores)
  • Comprehensive spectroscopic analysis (in cuvettes and on microplates)
  • Assay development and fluorescent imaging of live cells with confocal microscopy (e.g. FRET and FLIM) and super-resolution nanoscopy (e.g. STED and MINFLUX)
Keywords
  • molecular probes
  • fluorescence
  • bioluminescence
  • microscopy
  • chemical biology
  • biological chemistry
  • live-cell imaging
  • cell lines
  • assays
  • HTS
  • high throughput screening
  • free radicals
  • metal ions
  • proteins/enzymes
  • biomarkers
  • prodrugs
  • FRET
  • FLIM
  • MINFLUX
  • COVID-19

Affiliated laboratories:

Research activity

  • Preliminary research on chemical programming of fungi using Ramularia collo-cygni as a model. Miniatura 4 by National Science Centre (NCN),No: 2020/04/X/NZ1/01959
  • MultiGATE: dual-analyte responsive fluorescent probes for a real-time multi-parametric sensing in cellular models. FPS - HOMING 4 and additional FPS funding for research on COVID-19.
  • Synthesis and characterisation of conjugates of pH-responsive probes and triplex-forming oligonucleotides. NSC – MINIATURA 3
  • Small molecule tools to investigate local microenvironment of proteins. NSC OPUS-15
  • Masked bioluminogenic substrates for a selective imaging of the biochemical parameters of cancer. NSC – SONATA 13
  • EU-OPENSCREEN-DRIVE: Ensuring long-term sustainability of excellence in chemical biology within Europe and beyond. H2020 - H2020-INFRADEV-2018-2020
  • Project of Polish Platform of Screening Infrastructure for Chemical Biology (POL-OPENSCREEN) which is a part of European consortium EU-OPENSCREEN (European Infrastructure of Open Screening Platforms for Chemical Biology European Research Infrastructure Consortium). Funding from the Polish Ministry of Science and Higher Education (MNiSW)
  • POIR.04.02.00-00-C004/19 NEBI – National Centre for Imaging in Biological and Biomedical Sciences. POIR – action 4.2. Development of modern research infrastructure for science (H2020).
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