Department of Nucleic Acids Bioengineering

dr hab. Anna Pasternak

Dr. habil. Anna Pasternak

Head of Department

apa@ibch.poznan.pl
ext. 1279

Researchers:

Dr. Jolanta Lisowiec-Wąchnicka BEng.
adiunkt

Dr. Weronika Kotkowiak
adiunkt

Research Projects

Research area
  • development of novel molecular tools and devices based on nucleic acids and their analogues,
  • chemical synthesis of modified nucleosides and oligonucleotides,
  • thermodynamic and structural (CD, TDS) analysis of various nucleic acids motifs
  • (e.g. G-quadruplexes, triplexes, i-motif),
  • thermodynamics of siRNAs,
  • regulation of siRNA strand selection by RISC complex using modified nucleotides,
  • allele-selective degradation of mRNA via chemically modified siRNAs,
  • alternative splicing regulation in cancer cell lines,
  • development of potent anticoagulants based on modified oligonucleotides,
  • in vitro studies of chemically modified G-quadruplexes and their antiproliferative properties,
  • chemical synthesis of novel dyes with potential photodynamic activity.

As co-investigators:

  • in vitro studies of dye-oligonucleotide conjugates as useful tools for photodynamic therapy (Principal investigator: Michał Kotkowiak, PhD, Faculty of Technical Physics, Institute of Physics, Poznan University of Technology, Poznań, Poland),
  • studies on therapeutic potential of antisense oligonucleotides in gene silencing process (Prof. Jesper Wengel, University of Southern Denmark, Odense)
Keywords
  • modified nucleosides,
  • modified oligonucleotides,
  • G-quadruplexes,
  • triplexes,
  • i-motif,
  • alternative splicing regulation,
  • RNA,
  • DNA
  • aptamers,
  • therapeutic oligonucleotides,
  • diagnostics,
  • theranostics,
  • cancer treatment,
  • siRNAs,
  • nucleic acids thermodynamics,

Research activity

  • Foldback triplex-forming oligonucleotides as novel tools for G-quadruplex unfolding and selective inhibition of c-Myc oncogene transcription, NSC OPUS research grant: 2020/37/B/NZ7/02008, Principal Investigator: Anna Pasternak
  • Novel bispecific G-quadruplex conjugates as potential anticancer agents, NSC PRELUDIUM 18 research grant: 2019/35/N/NZ7/02777, Principal Investigator: Carolina Roxo
  • Structural determinants of anticancer properties of G-rich oligonucleotides, grant NCN: UMO-2017/25/B/NZ7/00127, Principal Investigator: Anna Pasternak
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